| 1. | Like mrna, both trna and rrna are transcripts of chromosomal dna .
TRNA及rRNA同mRNA一样，都是染色体DNA的转录产物。 |
| 2. | Pcr analysis and southern blotting all confirmed that the ced - 9 gene has been integrated into the chromosomal dna of these transgenic plants
对所得到96株再生苗进行pcr检测，结果表明，阳性苗比例为34 。 |
| 3. | S . tenebrarius chromosomal dna , partially digested with sau3al to yield fragments of 6 ~ 15kb , was ligated with vector pij702
提取黑暗链霉菌h6总dna ，经sau3ai不完全酶切、凝胶电泳，收集6 15kb片段连接到载体pij702 。 |
| 4. | It confirmed that ibdv vp2 gene was integrated into nuclear chromosomal dna by pcr . pcr positve plants were double checked for incorporation of the recombinant gene by southern blots
在转基因组织生长成植株的过程中， vp2基因随着植物细胞的生长而得到表达。 |
| 5. | Sequencing analysis showed that the sequence of endoglucanase fragment exhibits 35 % homology with b . subtil is chromosomal dna ( from glyb to apre ) , and 27 % homology with bacillus sp
将此重组质粒phchi转化巨大芽抱杆菌b . megateriumap25 ，得到两株转化子，分别命名为p25113一9 ， p25113一10 。 |
| 6. | Bhattacharyya np , basu p , das m , et al negligible male gene flow across ethnic boundaries in india , revealed by analysis of y - chromosomal dna polymorphisms [ j ] . genome res . 1999 aug ; 9 ( 8 ) : 711 - 9
李冬娜、应大君、区采莹，等.中国海南岛黎族人群y染色体上四个微卫星基因座的多态性研究.中华医学遗传学杂志[ j ] . 2003 ; 1 : 1 - 3 |
| 7. | A fusion - trascriptional library was then constructed by inserting sau3ai - partially digested chromosomal dna from 7653r into bamhi cloning site of phn127 . a group of constitutive - expression fusions with different fluorescent strength were obtained
将7653r的总dna经sau3ai部分酶解并克隆到phn127上，获得的融合子库，从中筛选到gfp组成型表达的具有调控活性的dna片段。 |
| 8. | Four colonies of transformed e . coli dh5 a with clear hydroiyzing zone on the chitin agar were obtained . the gene fragment in these isolates was identified by the methods of plasmid processing . dna sequencing analysis showed that sequence homology between pcr fragment and chromosomal dna of b . subtilis from 2599451 to 2812870 was 85 % , and was 30 % between the fragment and the genes encoding for chitinase of bacillus ( including b . subtilis ) in genebank
测序并序列比较结果表明该基因片段同已发表的枯草芽孢杆菌几丁质酶和内切葡聚糖酶编码幕因的克隆及重组芽抱杆菌的构建glyb一apre之间的同源性是最高的，为35 % ;同bacz ’了了ussp . bp23ce1b 、 b . p朋刀us内切葡聚糖酶和b . pol理vxap一1 ， 4一内切葡聚糖酶的编码基因的同源性只有27 % 。 |
| 9. | Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates . the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2 , w5 t1 and t7 . these pcr amplified fragments were cloned , and sequenced
首先利用芽孢杆菌中芽孢的抗热性将土壤溶液在100沸水中煮10 - 15分钟，然后用选择性无氮培养基进行初筛得到29株菌落形态不同的菌株；接着用固氮酶结构基因nifh的特异性引物对这29株菌进行pcr扩增，结果表明其中7个菌株具有nifh基因，这7个菌株的编号依次为： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。 |
| 10. | Strain pseudomonas psuedoalcaligenese ys1 was capable of producing phas containing monomer of hb and mcl has in certain medium . phacl and phac2 , two key polyhydroxyalkanoates polymerase genes of pha biosynthesis were amplified and cloned from chromosomal dna of pseudomonas psuedoalcaligenese ys1 using pcr
本研究利用聚合酶链式反应( pcr )技术，从p . psuedoalcaligeneseys1染色体dna中扩增并克隆了调控短链与中链pha生物合成的两个关键酶基因： phac1 、 phac2基因。 |
